MersV1, Main, Exploration, bibRecord, 000A07

Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.

Identifieur interne : 000A07 ( Main/Exploration ); précédent : 000A06; suivant : 000A08

Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.

Auteurs : Kunse Lee [Corée du Sud] ; Hae Li Ko [Corée du Sud] ; Eun-Young Lee [Corée du Sud] ; Hyo-Jung Park [Corée du Sud] ; Young Seok Kim [Corée du Sud] ; Yeon-Sook Kim [Corée du Sud] ; Nam-Hyuk Cho [Corée du Sud] ; Man-Seong Park [Corée du Sud] ; Sang-Myeong Lee [Corée du Sud] ; Jihye Kim [Corée du Sud] ; Hun Kim [Corée du Sud] ; Baik Lin Seong [Corée du Sud] ; Jae-Hwan Nam [Corée du Sud]

Source :

Microbiology and immunology [ 1348-0421 ] ; 2018.

RBID : pubmed:30117617

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Baculoviridae (genetics), Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli (genetics), Gene Expression, Humans, Immunologic Tests (methods), Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (immunology), Protein Binding (genetics), Protein Binding (immunology), Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Receptors, Virus (immunology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells, Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (immunology), Viral Matrix Proteins (genetics), Viral Matrix Proteins (immunology).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Receptors, Virus, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins.
- chemical , isolation & purification : Antibodies, Viral, Antigens, Viral.
- chemical : Antibodies, Monoclonal.
- diagnosis : Coronavirus Infections.
- genetics : Baculoviridae, Escherichia coli, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- immunology : Coronavirus Infections, Middle East Respiratory Syndrome Coronavirus, Protein Binding.
- methods : Enzyme-Linked Immunosorbent Assay, Immunologic Tests.
- virology : Coronavirus Infections.
- Animals, Gene Expression, Humans, Protein Interaction Domains and Motifs, Rats, Rats, Wistar, Reproducibility of Results, Sensitivity and Specificity, Sf9 Cells.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.

DOI: 10.1111/1348-0421.12643
PubMed: 30117617

Affiliations:

Le document en format XML

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<author><name sortKey="Kim, Jihye" sort="Kim, Jihye" uniqKey="Kim J" first="Jihye" last="Kim">Jihye Kim</name>
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<term>Antibodies, Viral (isolation & purification)</term>
<term>Antigens, Viral (immunology)</term>
<term>Antigens, Viral (isolation & purification)</term>
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<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (virology)</term>
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<term>Escherichia coli (genetics)</term>
<term>Gene Expression</term>
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<term>Immunologic Tests (methods)</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Protein Binding (genetics)</term>
<term>Protein Binding (immunology)</term>
<term>Protein Interaction Domains and Motifs</term>
<term>Rats</term>
<term>Rats, Wistar</term>
<term>Receptors, Virus (immunology)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
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<term>Anticorps antiviraux (immunologie)</term>
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<term>Anticorps monoclonaux</term>
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<term>Antigènes viraux (isolement et purification)</term>
<term>Baculoviridae (génétique)</term>
<term>Cellules Sf9</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Escherichia coli (génétique)</term>
<term>Expression des gènes</term>
<term>Glycoprotéine de spicule des coronavirus (génétique)</term>
<term>Glycoprotéine de spicule des coronavirus (immunologie)</term>
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<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (virologie)</term>
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<term>Protéines de la matrice virale (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Rat Wistar</term>
<term>Rats</term>
<term>Reproductibilité des résultats</term>
<term>Récepteurs viraux (immunologie)</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA ()</term>
<term>Tests immunologiques ()</term>
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<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Matrix Proteins</term>
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<term>Antigens, Viral</term>
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<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Escherichia coli</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Liaison aux protéines</term>
<term>Protéines de la matrice virale</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Infections à coronavirus</term>
<term>Liaison aux protéines</term>
<term>Protéines de la matrice virale</term>
<term>Protéines recombinantes</term>
<term>Récepteurs viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Middle East Respiratory Syndrome Coronavirus</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Immunologic Tests</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Gene Expression</term>
<term>Humans</term>
<term>Protein Interaction Domains and Motifs</term>
<term>Rats</term>
<term>Rats, Wistar</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Sf9 Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux</term>
<term>Cellules Sf9</term>
<term>Expression des gènes</term>
<term>Humains</term>
<term>Motifs et domaines d'intéraction protéique</term>
<term>Rat Wistar</term>
<term>Rats</term>
<term>Reproductibilité des résultats</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA</term>
<term>Tests immunologiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV.</div>
</front>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
</list>
<tree><country name="Corée du Sud"><noRegion><name sortKey="Lee, Kunse" sort="Lee, Kunse" uniqKey="Lee K" first="Kunse" last="Lee">Kunse Lee</name>
</noRegion>
<name sortKey="Cho, Nam Hyuk" sort="Cho, Nam Hyuk" uniqKey="Cho N" first="Nam-Hyuk" last="Cho">Nam-Hyuk Cho</name>
<name sortKey="Kim, Hun" sort="Kim, Hun" uniqKey="Kim H" first="Hun" last="Kim">Hun Kim</name>
<name sortKey="Kim, Jihye" sort="Kim, Jihye" uniqKey="Kim J" first="Jihye" last="Kim">Jihye Kim</name>
<name sortKey="Kim, Yeon Sook" sort="Kim, Yeon Sook" uniqKey="Kim Y" first="Yeon-Sook" last="Kim">Yeon-Sook Kim</name>
<name sortKey="Kim, Young Seok" sort="Kim, Young Seok" uniqKey="Kim Y" first="Young Seok" last="Kim">Young Seok Kim</name>
<name sortKey="Ko, Hae Li" sort="Ko, Hae Li" uniqKey="Ko H" first="Hae Li" last="Ko">Hae Li Ko</name>
<name sortKey="Lee, Eun Young" sort="Lee, Eun Young" uniqKey="Lee E" first="Eun-Young" last="Lee">Eun-Young Lee</name>
<name sortKey="Lee, Sang Myeong" sort="Lee, Sang Myeong" uniqKey="Lee S" first="Sang-Myeong" last="Lee">Sang-Myeong Lee</name>
<name sortKey="Nam, Jae Hwan" sort="Nam, Jae Hwan" uniqKey="Nam J" first="Jae-Hwan" last="Nam">Jae-Hwan Nam</name>
<name sortKey="Park, Hyo Jung" sort="Park, Hyo Jung" uniqKey="Park H" first="Hyo-Jung" last="Park">Hyo-Jung Park</name>
<name sortKey="Park, Man Seong" sort="Park, Man Seong" uniqKey="Park M" first="Man-Seong" last="Park">Man-Seong Park</name>
<name sortKey="Seong, Baik Lin" sort="Seong, Baik Lin" uniqKey="Seong B" first="Baik Lin" last="Seong">Baik Lin Seong</name>
</country>
</tree>
</affiliations>
</record>

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Serveur d'exploration MERS

Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.

Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus.

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